Basic protein and peptide protocols methods in molecular biology 1994th edition by john m walker editor isbn 13 978 0896032699 isbn 10 0896032698 why is isbn important isbn this bar code number lets you verify that youre getting exactly the right version or edition of a book the 13 digit and 10 digit formats both work. Basic protein and peptide protocols editors view affiliations john m walker book 336 citations 4 mentions 422k downloads part of the methods in molecular biology molecular weight estimation for native proteins using size exclusion high performance liquid chromatography. Methods in molecular biology john m walker series editor 32 basic protein and peptide protocols edited by john m walker 1994 31 protocols for gene analysis edited byadrian j harwood 1994 30. Comprehensive and easy to follow hplc of peptides and proteins methods and protocols offers every biologist and biotechnologist at all levels of expertise ready access to the powerful tools needed to develop and carry out peptide and protein separations and analyses today. The classical hydrolysis conditions to which all other methods are compared is liquid phase hydrolysis in which the protein or peptide sample is heated in 6m hydrochloric acid under vacuum at 110c for 18 24 h the various methods of hydrolysis described here are summarized in table 2
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